Conversations with Microbiome Labs

Course Content

1/13/25 Balance from Within: How Probiotics Are Transforming Vaginal Health with Alicia Galvin
12/17/24 Stool Testing: What You Need to Know with Alicia Galvin
59:28
10/24/24 The Female Microbiome: Clinical Insights and Practical Applications for Optimizing Women’s Health with Alicia Galvin
51:56
8/27/24 Immunoglobulins 101: How to Support Mucosal Immunity, Microbial Balance, and More with Sarah Ellis
1:07:12
7/30/24 Set the Foundation to Gut Health with Lacey Hall, MS, RD
59:22
7/9/24 Microbiome Foundations: Dysbiosis and the Gut-Organ Axis with Sarah Ellis
1:23:30
6/26/24 Food Sensitivities: FODMAP, Gluten and More with Lacey Hall
59:49
How to Build a Resilient Microbiome with Alicia Galvin 5/7/24
1:02:57
Pioneering Gut-Brain Axis and Digestive Support with Kara Siedman 4/9/24
1:01:03
Stress, Sleep, and the Impact of the Microbiome with Alicia Galvin 1/29/24
58:46
Feeling Zapped of Energy? Mitochondrial Rescue with Kara Siedman MBL 11/13/23
53:37
Acute and Long-Term Antibiotic Therapy Recovery with Sarah Ellis (antibiotic recovery) 10/18/23
1:05:02
Metabolic Conditions, Blood Sugar Balance, and Gut Health with Alicia Galvin (metabolic conditions)
57:23
The Forgotten Microbiome: Why Oral and Dental Health Matter and the guest expert is Dr. Haroldo Magarinos 8.21.23
1:00:13
The Gut-Brain Axis: Restoring the Gut for Better Brain Health and Mood with Kiran Krishnan 7.24.23
48:08
Weight Loss/Metabolic with Kiran Krishnan 6.27.23
59:38
New Research: Multiple Strains for Microbiome Support for Gut, Sleep & Mood with Kiran Krishnan 5/1/23
1:12:21
The Pediatric Microbiome: Gut Support Your Kids Will Actually Eat with Kiran Krishnan 4.17.23
59:49
K2: Beyond Bone and Heart Health with Kiran Krishnan 4.4.23
57:18
Spore-based Probiotics: A User's Guide with Kiran Krishnan
1:04:49
Maintaining (and Improving) Your Digestive Health Over the Holidays with Kiran Krishnan
58:54
Prebiotics and Probiotics with Kiran Krishnan
53:31
Vitamin D3/K2 — The Calcium Connection with Kiran Krishnan
49:12
The Deep Dive into Healing the Gut Lining with Kiran Krishnan
1:07:22
How Does Oral Health Impact Your Gut and Systemic Function with Kiran Krishnan
49:10
The Gut-Skin-Microbiome Matrix with Kiran Krishnan
56:52
The Thirteen Studies on MegasporeBiotics with Kiran Krishnan
1:22:14
The Role of Spore Probiotics in Gut Health
1:13:37
Microbiome Superpowers with Kiran Krishnan and Shivan Sarna
32:17
The Gut-Brain Axis and the Role of the Microbiome: Managing Stress Response and Sleep with Kiran Krishnan
Examining the Root Cause of SIBO - Going Beyond the Bloat With Kiran Krishnan
1:35:07
The Gut-Skin-Brain-Sleep Matrix and the "Psychobiome" with Kiran Krishnan
35:36
MegaSporeBiotic™
19:08
Microbiome Breakthroughs and the Future with Kiran Krishnan & Shivan Sarna
26:39
How To Diversify Your Diet Without Symptoms
18:55
How the Brain Is Impacted by Your Gut with Kiran Krishnan of Microbiome Labs and Shivan Sarna
1:16:44
Loving on Your Immune System and Staying Well This Winter (Recorded Oct 27, 2020)
1:44:19
How Does the Microbiome Control Your Immune System with Kiran Krishnan (recorded Sept 16, 2020)
1:00:06
Are Stool Tests Full of Cr@p? with Microbiologist Kiran Krishnan
1:15:11
3 -Step Total Gut Restoration Process
1:02:38
A Microbiologist’s Perspective on Small Intestine Bacterial Overgrowth
47:18
Immune-Gut Matrix: What You Need to Know Now with Kiran Krishnan and Shivan Sarna (Recorded Jan 28, 2021)
1:08:19

59:22

Hi. I'm Siobhan Sarna, and I'm the founder of Chronic Condition Rescue and SIBO SOS. And for the past, gosh, now 7 years, I have, more than that, I've really appreciated what Microbiome Labs does. And for the past 5 4 or 5 years, they have been really excellent at educating us here in this community. And today, it is no different.

We have Lacey Hall here, who I'm gonna really mess up her title, but executive scientific officer and educator. What's her own title, Lacey? Head of scientific affairs. Just I do the science. To okay.

That's that's it. Right? Here you go. So what we're gonna do today is talk about leaky gut and how to and how to get a resilient microbiome and what you can do to help overhaul your microbiome. So I'm gonna just quickly hand things over to her.

I know it's later than usual, so I'm gonna let her get started. The presentation is phenomenal, and I'm very, very excited about this. Head of scientific affairs. Very nice. Let's see.

Can you guys see my notes screen, or are you just seeing We see the notes screen. Okay. I have to unplug from the external monitor. I don't know how to Okay. Make it work that way.

Let's see. Let me stop share and reshare. Okay? Okay. Sure.

Sorry about that. Oh, no or yes? It's bigger. It's bigger. I think it's There we go.

Whether or not it's full screen, I don't know, but it looks good. It looks good. Alright. It's great to be here, everyone. Thank you so much.

Yeah. My name is Lacey Hall. I love talking about the gut microbiome. It's a huge passion of mine because it's such an integral part of root cause Allison. And that's what we're in the business of.

When you heal the gut, you're healing so many parts of the body. So today, we're Sarna talk about building resilience through the gut microbiome and we'll talk about a 3 step program called the Total Gut Restoration Program. And this is a way to really get the gut back on track. It's very foundational. So I will have to nerd out with you a little bit.

We'll throw in some sciency terms. If you need, some clarification, just please throw it in the chat some questions and I'll be sure to answer them at the end. And I'll try to keep it, fun and light with some quiz questions along the way too. But what she really meant was put them in the q and a box. Oh, sorry.

Put them in the q and a box. So Sarna, so sorry. Technical terms. Alright. So we think about a healthy gut microbiome, what are we talking about?

We know that a microbiome impacts our overall health. What does it need to do for us? We need it to produce a bunch of molecules called postbiotics. This comes from the foods that you're eating, especially foods like fiber. We need it to contribute to a well functioning intestinal barrier.

We want that barrier to be a reinforcement Sarna nothing can get through. We want it to have a low inflammatory load, meaning that it has, there aren't a lot of inflammatory organisms and pathogenic bacteria that are living there. We Sarna keep it in Allison. And we know that when we do this, we will build resilience so it will lead to less chronic diseases, especially that stem in the gut, but not necessarily just in the gut, really thinking about the overall health and wellness of our body. So quick quiz question.

What do you think is the average number of microbial species that are found in the gut microbiome? If you Sarna throw that in the can I say throw that in the chat? Throw that one in the chat. Right? Sure.

We're not. Sure. What do you think it is? I I asked some people a little while ago and they had some crazy answers. I'd love to see what you guys think.

Well, the answer is 3 to 500 species of bacteria microorganisms. So that number has gone down as our industrialized lifestyle and exposure to things like antibiotics has grown over the years, but that's a really good microbiome. Now I see a lot of stool tests that come across my desk with patients that have around 50, 75, or a100. That's pretty common, and we wanna get that number as high as possible. And I'll explain why through this deck.

So when we think about a healthy gut, what is a healthy gut? Let's create some, guardrails around this term. So we look at this picture here on the left, a healthy gut. Let's define it with just a few areas. We want high diversity.

So that number of bacteria that live within the gut, we want the highest number possible. That 3 to 500 is a great, goal. It's a hard one actually, but we want to get as many bacteria and organisms living there as possible. And we want high numbers of our protective strains. We call these our commensal organisms or our keystone organisms.

We want those organisms to produce something called short chain fatty acids. That's the SCFA, short chain fatty acids. Those are these amazing molecules that are created by these bacteria that play a role in your overall health. And then at the very bottom here, we want these well formed tight junctions. That means this little caterpillar that you see on the screen is actually your intestinal lining, and we want to have these cells glued together.

We don't want gaps formed or, coming up in them. We want everything healed and sealed and and linked together so that it prevents something called leaky gut. So we see these three layers here, the the bacteria, we see this blue area as our mucosal lining, and then we see our epithelial barrier, our intestinal cells. So those are the areas that we're gonna look to talk about, during this presentation. But dysbiosis and leaky gut is something that, presents in most patients that I see on a day to day basis.

What is dysbiosis? It's the opposite. It's a low number of the protective types of bacteria that we really want growing in the gut. And acrymansia, muciniphila, phycalibacterium praesnitzii, and different bifidobacteria groups. You don't have to know those.

We can just call them keystone species. They're our most protective groups. Low diversity, those are the patients whose stool tests come across my desk with the 50 to 75, number of bacteria living in their gut. We Sarna get them to that 300 to 500. This means that they're not gonna produce a lot of short chain fatty acids.

So there's not a lot of functionality. Your gut isn't communicating because that's how it communicates with other parts of the body. And then we have a break in this mucus barrier. That means that the bacteria in your gut are actually touching and interacting with your epithelial barrier, the cells of your intestinal lining. And we don't want that.

If you look at the picture, we can see that just like kind of surge or overtaking like a castle gate. We see this burst through this epithelial epithelial barrier into your own circulatory or bloodstream system, and it causes your immune system to freak out and this leads to inflammation. So when bacteria get into your bloodstream, this leads to bacterial toxins getting into your bloodstream and your immune system is like, this isn't supposed to be here. You're not supposed to be here. Get out of here.

So it leads to an inflammatory response. But your gut is very long. Your colon itself is 5 feet long. And if you have these little punctures all throughout that area, that little snowball of inflammation turns into something much bigger. Now what are some of these risk factors that lead to dysbiosis and some of those unfavorable characteristics in Dr.

gut? Medications like antibiotics and natural antimicrobial therapies. I'm not saying we don't need them, but we just need to like a SIBO treatment where it's imperative to be on some antimicrobials and antibiotics here and there. It depends on the patient and the health care provider, but we see it all the time. Our message is not to skip them, but let's rebuild afterwards.

Infections that can be from foodborne illness, just general sickness, being exposed to things especially while traveling, excessive of alcohol, smoking, stress, travel. I'm definitely someone that fits into the travel category, so I have to take extra care of my gut. I travel a lot for work. Saturated fats and the standard American diet that would be compromised of high calorie, high sugar, high fat, of course, the fast food. And while you might know that we're not supposed to eat these things, of course, the majority of Americans do.

So this leads to big changes in the microbial landscape. Different toxins in our own environment and our household items like cleaning products Sarna then also pesticides and residues that we find on fruits and vegetables if we're not able to choose organic. So glyphosates play a huge role in the composition of the types of bacteria living in our gut, and then, heavy metals can play a role too. Lack of sleep. Oh, I have a baby.

That's part of mine as well. Intense exercise as well. Even though it's something good, it can lead to changes in the gut and a leakiness and a permeability within that gut lining. So I'm sure some of us can check off a few areas here that, we may have strengths and weaknesses in, but it can all lead to changes in the overall microorganisms. So going back to this picture, our main issue and the main thing that we're looking to control when we're thinking about the gut and dysbiosis is something called lipopolysaccharide or LPS for short, and lipopolysaccharide comes from bacteria in our gut.

And even if you have the most perfect and beautiful microbiome, you still have lipopolysaccharide there. Lipopolysaccharide is highly inflammatory and highly toxic to the human body. We all have some of it there. Too much of it. So what we can we can implement different therapies to control that.

But what happens if we do have this leakiness and permeability to the gut lining where bacteria is able to enter the bloodstream, this is going to, again, cause the immune system to, have a freak out moment. That's the that's the technical term, I promise you. So lipopolysaccharide is gonna trigger the immune system, that leads to an inflammatory response. So we don't want that to happen. We want to heal and seal these breaks in the foundation, and change the types of organisms living there so we don't have too much lipopolysaccharides circulating.

What is lipopolysaccharide? If you look at the small image on the left, we see a little drawing of a bacteria. Lipopolysaccharide Dr. LPS lives in the outside membrane of the bacteria of certain types of bacteria called gram negative. So again, it's naturally present.

But this toxin, is very, immunogenic. It's very inflammatory to the body. So we just wanna make sure there isn't too much of it occurring. Now if you Sarna get technical, for those of you that really like to get into the weeds, into the nitty gritty, Gram negative bacteria belong to a group called proteobacteria. And proteobacteria is where our pathogenic organisms like E.

Coli and salmonella. I'm sure you're familiar with those those types of organisms, but there's plenty other that live in that category as well like H. Pylori. They all contain lipopolysaccharide in their cell wall. Now with metabolic endotoxemia, when this, reaction occurs essentially in the body, it's triggering the immune system.

Now your immune system and microbiome are very intimately connected. Some people may have heard the term that 70% of your immune system lives in your gut, and that's true. It's true because on one side of that intestinal wall, you have bacteria. On the other side, you have your immune system. Why?

It's a training camp for bacteria because then your immune system is learning how to figure out who's a friend and who's an enemy. So it's very important. So your immune system lives there. Also, we don't wanna allow bacterial infection. So anyway, metabolic endotoxemia is is when too much lipopolysaccharide is entering the bloodstream, and it's triggering this immune response causing low grade inflammation.

So we think of this snowball that's slowly growing, and is driven by the bacteria and lipopolysaccharide. It's estimated to affect about a third of the population, but I would argue that it's probably much, much higher. We just don't have a really good way of capturing it. And it's characterized by a spike in lipopolysaccharide, from bacteria, toxins getting into the bloodstream, and this happens actually after consuming food, typically after a really high fat diet, especially one that's high in saturated fat. Oil was the highest, I believe, that spiked the the LPS serum endotoxin after eating.

That doesn't mean you need to avoid it. We just need to make sure that you have a really strong and resilient gut to prevent that LPS spike. And we know that when there's elevated inflammation, this can impact a lot of things like your triglycerides and and your insulin production and response as well. So there's a lot of, clinical scenarios in which the LPS, lipopolysaccharide, is linked to different conditions. So I'll just show you a few of those right now.

So we know that this LPS, metabolic endotoxemia, is linked to obesity and insulin resistance. We know that it's linked to type 2 diabetes and, in many ways actually, and it's interesting how the gut plays such a role over metabolic health. Everything with semaglutide, Ozempic, GLP one agonists, that all has to do with the gut. So the gut becomes this this gateway to so many different things. We know that LPS plays a huge role in overall gut brain connection, anxiety and depression and other mental health conditions, and it's able to actually cross the blood brain barrier so much that we actually see LPS, embedded and enriched in different areas of the brain of Alzheimer's disease patients since it's able to cross that barrier.

We see a disruption in female reproductive health with too much circulating LPS in the bloodstream, and we see it as a trigger and contributor to autoimmune diseases and, motility issues as well. We see LPS playing a role in gastric motility, influencing things like gastroparesis where food isn't leaving your stomach, loss of appetite and nausea. It plays a role in a lot of things, but that's because our gut is that gateway. It is a portal, and I think that's the beauty of it all. When we start to fix the gut and we change the type of bacteria that are living in the gut, then we can see, a triple effect of other, systems functioning better and how we're feeling on a day to day basis.

So this microbiome impact, we call this gut organ axis. So if you look at the picture on on the right, when you run along all these pathways, we see direct links from the gut to adipose tissue, the way in which it influences the fat tissue within our body, the gut liver access, the gut plays a role in how well we detoxify, and a lot of people wanna go on detox programs, but if you're neglecting the gut, we're not gonna have as good of a of a detox. The gut heart access, heart health and cardiometabolic health is really important. Gut kidney access, gut brain access, skin and bone, just to name a few, and there are many others. So, again, when we're we're laying the foundation, getting to root cause medicine, we're we're really helping the patient and helping you feel better on a day to day basis.

True or false? You have more bacteria in your gut than actual human cells. You think about that. Your entire human body. Do you have more bacteria in your gut?

I mean, more bacterial cells in your gut than human cells. True or false? The answer to that is true. You actually have up to 100 of trillions of gut bacteria, while you have roughly 37.5 trillion human cells. So sometimes it's hard to fathom how is that possible because we're obviously a huge being, but bacterial cells are are much smaller, so that's that's one of the reasons, but it's hard to fathom.

In some ways, we are more bacteria than human, And in fact, there is no sterile place in your body. There's a unique bacterial fingerprint and blueprint in every single area. Even your ear canal canal has its own microbiome, that is very different from your gut microbiome. So our goal is to get you to this point here where you have this resilient and productive ecosystem, this thriving rainforest that's residing in your gut filled with different species of bacteria rich in nutrients that's alive and working together and in balance. But what we find is most people are here, kind of a desert zone where there aren't a lot of bacterial species.

It's missing the mark. It's unhealthy. It's not optimal for growth or wellness, and you're not gonna have those strong accesses connecting the gut to all the different places in the body. And then this is one that's just unattended. This is similar to looking at our, the gut barrier, the different cells.

When you've got cracks in that pavement and bacteria and bacterial toxins like LPS are seeping through, then that's going to cause a lot of inflammation in the body. So what can we do? So let's talk about total gut restoration program. So the first stop in reconditioning the gut is with our flagship product. It's called megasporbiotic, and this is gonna help change the type of microbes living there, and it's gonna help increase those really healthy species called keystone species.

So there's a lot of probiotics on the market. I mean, that's an understatement. So traditional So traditional probiotics are typically lactobacillus and bifidobacteria, and they can work, but not in the way than which we want. We wanna change that entire ecosystem so it looks like a rainforest where bifidobacteria and lactobacillus are really just 2 little species, right, in that entire rainforest that we wanna impact. And most likely, it's not gonna survive the gastric, your gastric juices to really reach the colon, which is 15 feet away from your mouth.

It's a long journey. It's a long, hard journey. So we need to rethink the traditional probiotics. We can use bifidobacteria and lactobacillus, but for different things. So let's forget the old school method and think to now.

So we we want to take you on a paradigm Shivan. We change your mindset. So spores, bacterial spores Mark what we use in megaspore Briotic. They can remain dormant in this little hibernative state for up to centuries. They can withstand very harsh environments like your stomach acid, your digestive enzymes, and your bile to reach all the way to the colon so that they can go to work.

They can survive in oxygen and without oxygen, so they're very resilient, and they've been known to work in the human body and make changes to the gut microbiome, and we have a lot of research behind that. So it's the first 100% spore based probiotic, offered to to you today in the United States. We have 5 strains of bacillus bacteria that each have a different function, and I'll I'll go over some of those functions. You don't have to refrigerate it. You don't even have to keep it in the capsule.

You can dump it into a little bit of yogurt and take it if you don't like taking pills. It survives digestion, has a long shelf life, and we take that product and we put it in all sorts of clinical studies. Because we Sarna show you what we're using in the studies is the same product that you're going to pick up on store shelves because that's very important to us that, it translates from research to you, to your home. So the 5 different strains are listed here. They're all bacillus strains.

We have bacillus subtilis hu 58. What does this do? It creates its own natural antibiotics, And that may sound counterintuitive, like, hey, we don't Sarna do that, but they're selective. It's really interesting how these antibiotics will attack the more pathogenic organisms, the organisms that, contain LPS without disturbing commensal and keystone species. So they're it's smarter than the technologies that we can create in a lab.

We're harnessing the power of nature. It produces vitamin k 2. K 2, especially in women, is super critical to bone mass and density, in women, is super critical to bone mass and density, along with a Allison other things, but especially bone mass and bone density. Vitamin K2 puts calcium where it needs to go. That's its main job.

It's really important for immune cell development as well. Bacillus coagulans is really important for supporting the immune system, especially in gastrointestinal disorders. Bacillus clausii is really good for respiratory health. We see this as a benefit, especially in children with asthma. Bacillus, like caniformis, can produce its own antibiotic, bacitracin, which you probably use over the counter for topical cuts.

It can help improve protein digestion. It creates enzymes, and it produces b vitamins. And then last but not least, Bacillus Indicus produces antioxidants like carotenoids, lycopene, astaxanthin, beta carotene, and lutein that you can absorb, so then it's almost functioning as a gut microbiome multivitamin, and so it's helping to feed other bacteria in that environment. So what this does is it's feeding and supporting that entire rainforest ecosystem for those plants to grow, or bacteria, but in our rainforest Sarna algae plants. So it's not about these bacteria.

It's about supporting the environment and cleaning it up, and that's what we're doing with Megaspor Biotic. So our first study was, conducted in 2017, and we wanted to look at if you took Megaspor could we lower lipopolysaccharide, LPS, that that little sinister toxin coming from gram negative bacteria. So what did we do? We recruited college students with, very easily by offering them pizza and a little bit of money because college students will do a lot for very little. So we had 75 college students come to our research center and we said, hey, eat all of this thin crust cheese pizza.

We're gonna measure your blood for lipopolysaccharide, and we're gonna send you home with some probiotics Sarna placebo, which was a rice capsule. So the subjects ate the thin crust pizza. We measured their blood. We found that 5 hours after they ate, we see this big surge in lipopolysaccharide. So we were doing a real time analysis.

We were getting their blood and they were testing their blood right away. So we saw this big surge in, lipopolysaccharide, told the students to go home, take the probiotic And we and we found that the spore treatment, the ones that were taking megasporbiotic, there was a 42% reduction in lipopolysaccharide just from taking the probiotic because we were healing the gut. We were filling in those holes, healing the permeability, the holes and the gaps within the gut, and we were changing the types of bacteria there. So when we lower it by 42%, we're lowering our overall inflammation. We're making that very important change.

We also saw an increase in the placebo group, the group that was taking the rice capsule. Who knows why? We didn't tell them to change their diet, their lifestyle. These are college students. Of course, they're subject to staying up all night and partying and eating whatever they want, so, there were probably a lot of reasons for that, but we saw a protective effect without having to do any other behavior change.

Give me a moment. Now today, we have a lot of research supporting megasporbiotic and we tried to put it in a lot of different clinical studies and models. We find that, yes, it lowers lipopolysaccharide by 42% in 30 days. We find that it increases microbial diversity, meaning we can get more bacteria living there, a more robust and stronger microbiome. Remember, we want between 305 100, not 50 to 75.

We could increase short chain fatty acids. We can lower blood triglyceride levels without changing the diet. We can help protect the liver and stimulate antioxidant production in the liver. We can improve IBS symptoms Siebecker or the same as antibiotics, and we can improve the immune response during something like a C. Diff infection.

Those are just some of the clinical studies and outcomes that we have found. So another pop quiz question, megasporbiotic has been shown to, a, oh, I didn't it's supposed to say reduce LPS. Oh, reduce LPS. It does say that. Reduce serum triglycerides, reduce IBS symptoms, reduce CF infections, or e, all the Shivan.

And, of course, our answer is e, all of the above. We wanna show the diverse applications of the microbiome, not megaspore. We wanna show that when we heal the gut, we heal other areas of health, and so this is an example of that. So now we Sarna reinforce what we're doing. Megaspore, think of it as the seeds in the garden, but mega prebiotic, a prebiotic fiber is the fertilizer.

So we gotta give those bacteria the food they like to eat, and thankfully, we know what they like to eat. And when we feed bacteria the things they wanna eat, they create these chemical messengers called short chain fatty acids. They're kind of like the way in which your body communicates with your gut. We've got all these accesses, right, that we're talking about, gut liver, gut brain, gut heart. Well, the way in which those systems communicate is with these short chain fatty acids.

So So there's 3 primary ones, that we talk about, butyrate, propionate, and acetate, and all are very important, but butyrate is arguably the most important. So when we have a lot of butyrate production, this creates a very healthy environment, but each has its own characteristics and and things that it's doing within the gut. So very important. And another and one of the very important aspects of short chain fatty acids is it keeps the gut a little more acidic. We want your gut, the environment, to be more acidic because then it prevents pathogens from growing.

So when you have, prebiotic fibers feeding those healthy bacteria, it's helping to grow keystone species, those very important organisms that play in the same place. It doesn't work. They're mutually exclusive, which is great because if we just focus on supporting the keystones, naturally, the pathogens would just make their way out. They don't like this type of environment where it's where it's a little more acidic. It supports other beneficial gut micro microorganisms and it supports neurological health and neurotransmitter health, like GABA production, buddhaion production, which is a really important antioxidant that's produced in the liver, and it's associated with lower levels of obesity, diabetes, liver disease, cardiovascular and metabolic health, inflammation, gastrointestinal disorders, asthma allergies, and chronic fatigue.

So supporting keystone species is a gateway to supporting overall microbiome health. It's foundational. So we can feed those keystone species with the foods they like, and they like fiber. And of course, you can get fiber in your diet. You do not need to supplement.

But this is a way to get there faster and to make sure you're giving your, keystone species the right fibers. So I ask I ask people sometimes, do you have money or time or do you have both? Because food takes a lot of time, the preparation and the planning, and if you wanna cut it short, you can use a supplement to get there faster, and it is very effective. So this is a blend of 3 functional fibers, xylo oligosaccharide from Sarna cob, fructooligosaccharide from kiwi, and galactooligosaccharide from cow's milk. Now pause before you blow up the q and a about corn and cow's milk Because non GMO corn cob is, it's actually the cob portion.

We're not looking at the proteins in corn. We're looking at the fiber portion. So most people have sensitivities to corn protein or milk protein. We're at we're taking out the protein portion and only using the fiber portion, so it's different. K?

Okay. Good good good call. Yes. Always get that question. That's number 1.

And the results speak for themselves both. So when we look at what we do when we combine megaspore with megapre, we see improved microbial diversity. We see improved keystone species like Bifidobacteria, lactobacillus, the Calibacterium prausnitzii. We see a reduction in pathogens and a huge increase in short chain fatty acids, 80 to 140 percent just in 4 weeks. So I'd argue that it's very, very good.

And no fear around the corn cob and the the milk, carbohydrates that we we add in there. It's keto friendly. It's not gonna spike your blood sugar. I said carbohydrate, but it's it's a fiber. Okay.

True or false? Prebiotic fibers feed pathogenic bacteria. So prebiotic fibers are for those oligosaccharides that I talked about. The answer is false. We know that your pre bi, your pathogens like to eat fat and protein and sugar and all sorts of other things.

Doesn't mean you shouldn't have fat or protein. We just don't need to have, you know, 300 grams of protein a day. It's hard for your kidneys. It's it's a load for your, microbiome. It's going to lead to more inflammation.

So we know what your bacteria like to eat, and your keystones love oligosaccharides. Now 3rd step in this protocol is to rebuild that mucosal barrier. So we wanna improve the mucus layer because that's gonna help separate things. Let me just show you a picture. So here is a electron microscopy photo of the gut microbiome.

Here at the top, this looks like sprinkles or confetti. This is where your gut microbiome is. Those are all of your beautiful bacteria all lit up looking very pretty. Then we have our mucus barrier, our mucin 2 layer, and this barrier is separating your gut microbiome from the epithelial lining, the cells lining your intestinal tract. So very important to keep everything separated out.

We don't want the bacteria interacting, putting all that pressure, so we wanna keep that mucus layer really built up. And people also wanna skip that step and, it can often be the most important step for stop the inflammation. So that thick barrier is really important for maintaining, acting as a line of defense, keeping things separated. It helps to, support the immune system with signaling molecules, and it's produced by cells in the intestinal lining called goblet cells. They produce the mucus.

And your healthy groups of bacteria play a role in this too, so that's why having a really healthy microbiome is important. Pathogens like to eat mucus. They eat it up, like, and they they break it down and that's why some people suffer from such thin mucus barriers, whereas your healthy groups of bacteria, the keystone species, specifically one of them called acromancia, go on the top of that mucus barrier like Pac Man and they eat that top layer of mucus, but then it allows the body to regenerate a new layer of mucus. It's a positive thing. So when we harness the power of those keystones, we can, have a much healthier gut.

K? So we have a product we have a couple of products. 1 is called IgG 2,000 Sarna another called megamucosa. Both of them contain an ingredient called, bovine immunoglobulins. Now these come from cows.

There's no dairy at all. It's not a dairy product, but we can take out these immunoglobulins that's part of the cow's immune system to help bind, bacterial toxins like lipopolysaccharide. So we Sarna bind it up like a sponge. Think of it as a bacterial toxin sponge that's soaking it up soaking it up. So if we use this, we find that 45 days utilizing the serum bovine immunoglobulins, SBI, reduced dietary endotoxin, that's LPS, no matter if you took 1 gram a day, 2 grams a day, or 4 grams a day.

Now patients find really good relief in taking IgG 2 1,000 especially if they suffer from IBS or, looser stools, more diarrhea. What IgG does, again, it's soaking up that LPS and other bacterial toxins, so it's telling it's giving your immune system a break. We use it as passive immunity. It's telling your immune system, okay. You can relax.

I got this. So it comes in, to help support any patient that has Crohn's or IBD. It gets really good relief from this. Now, serum bovine immunoglobulins, they help to bind a bunch of different toxins, but they also help to increase that short chain fatty acid production too. So you're getting a lot of functions.

Megamucosa also contains polyphenols. Polyphenols are are like antioxidants, but this helps to lower inflammation, increase short chain fatty acid, acid production as well, which is really helpful for that overall environment. And then the last ingredient that's found in this product are just amino acids, but we've selected 4 really important amino acids that help to increase mucus production. So these 4, amino acids listed here, threonine, serine, proline, and cysteine, can increase colonic mucin production by 95%. So, again, if we restore that mucus barrier, we can keep things separate.

We can reduce, LPS from even entering into the bloodstream. K. So my last pop quiz question of the day oh, I didn't put the the animation on there. We we know the answer. True or false?

You can have a healthy gut without having a healthy mucus mucosal barrier, and the answer is false. The mucosal barrier, oh, I think my text got cut off. Mucosal barrier is super important for just separating the two environments, but it also plays a role in immune signaling, immune health, and and the way in which the immune system can selectively grab bacteria and sample it. So it has a lot of functions that we need to pay attention to. So when we think about the 3 layers, if we break down a very complex world by the gut microbiome, it is complex, but if we just focus on these 3 foundational layers, then we can, really do a lot to heal the gut and get your your feeling back on track.

So if we start with step 1, megaspore. Let's change the type of bacteria that are living in this environment. We can increase microbial diversity and short chain fatty acids when we layer in mega prebiotic, we're giving it the food that it wants to eat. And then step 3, let's rebuild that mucosal barrier so that, these bacterial toxins aren't entering the bloodstream and causing havoc and an inflammatory response in the body. So we can take a very complex role by the gut microbiome and simplify it into 3 easy steps.

So who should take, or or follow a total gut restoration program? It's a great way to reset the gut overall, but the gut damaging factors that I listed before, if somebody was on antibiotics a lot, it's really good to do 1 or 2 rounds of it to to get them back on track. Patients that have eczema, allergies, food sensitivities, traveling a lot, have, of course, digestive issues like IBS, IBD, bloating, gas, and digestion, constipation, are just some of the the ways. Now if you like to do something like a a liver detox protocol, something like that, it's really good to start by supporting the gut first. You'll have a much better, support of the liver if the gut is functioning properly.

So start with the gut. It becomes ground 0 for a lot of, treatments. How do we do it? We start with megasporbiotic. Now we wanna do products 1 at a time because when we're changing the gut microbiome, you may feel those changes, and they're not always the best changes, so we wanna be careful there.

So megasporbiotic, first, we start with week 1, one cap every other day. Week 2, one cap daily. Week 3, try 2 caps a day. Now if any time you have a change in bowel habits, if you're feeling bloated, just go back a bit. You know, pull back.

For some people, one cap is too strong. We start with a half cap every other day or a quarter cap. It's not very often now. Maybe 10% of people. Now while still on megaspore, we layer in the megapre.

Again, this is the fertilizer that we have that is going to help support the growth of those keystone species. So you can do capsules. We also have a powder. I prefer the powder, but I just don't like a bunch of caps a day. So week 5 and through 6 to 8, do the mega pre, you layer it in.

And then step 3, megamucosap, rebuilding the mucosal barrier. Don't neglect the mucosal barrier. And you can do this in a powder form or a capsule form as well. So at step 3, you're doing all 3 products at the same time. It takes about 12 weeks, but we find that people have really good success just after those 12 weeks.

A maintenance program, we get this question a lot. Do you have to be honest forever? Absolutely not. Our main goal is to honestly heal you so you don't need any products. That's our our main goal, which is why we've committed to so much research, but, you know, we can't live in a bubble either, and it's hard to live a completely toxin and organic and stress free life, so doing a small maintenance can be helpful.

You can reduce taking megaspor to a couple of times a week and then reduce taking megabucosa and megapre as well, But there's always different cases for every patient that we have to be cognizant of. But a lot of people like to kick off the new year with a TGR protocol just to, you know, get your gut back on track. So let's summarize a little bit. Total gut restoration. We want to recondition with megasporbiotic.

We're planting the seeds to revitalize this garden. Shivan we do this and we create We're planting the seeds to revitalize this garden. When we do this and we create a better foundation, we can lower lipopolysaccharide entering into the bloodstream by 42% in just 30 days. We can increase short chain fatty acid production, and we're specifically growing our keystone species. No other probiotic on the market can say that, so we're very proud of that.

We wanna reinforce these changes with our mega prebiotic fertilizer. We wanna specifically feed these keystone species. Give them the food they want. We know what they like because this will help those keystone species grow. And the third step is megamucosa.

We build the mucosal barrier. We want that thick and robust mucosal barrier separating that microbiome from your intestinal lining, and we can bind toxins with the immunoglobulins. We can increase mucus production with the amino acids, and we can we can get more short chain fatty acid production with the polyphenols. So we've got a lot of different functions and just a couple of or 3 products that go a long way in just 12 weeks. Alright.

Thank you, Lacey. Let's put some love into the chat for Lacey. That was fantastic. Really thorough, really clear. I have tons of questions for you from our audience.

Thank you all for your patience and, you know, your beautiful responses. But, pop some love into the chat for Lacey. What was your big moment? This is a chat request. And then also in the chat is the 15% off your entire Microbiome Labs cart.

You can register for an account. It's all written out right there. You have to use patient code direct, direct code, Siebecker SOS, it's spelled for you. And then at checkout, use the coupon code no bloat 15. Now I have something else.

It's 6:12 EST. You know, I like to throw in, you know, they call them fast action bonuses. So for people who are doing something, with between now and we'll do 715. We do have something where you send proof of purchase to us at info atsibosos.com. And doctor Leonard Weinstock is a phenomenal gastroenterologist, and he did a presentation for our community on leaky gut.

And it's a masterclass that normally is $59. If anybody makes one purchase, whatever you want, before 7:15 EST and sends us the proof of purchase tonight, not tomorrow, you have some proof of purchase, then we will have a page set up where you can get this, master class. We'll send it to you, and I'm Sarna show it to you very briefly. I pulled it up, and here is sharing screen, and, then we'll get to your questions. Sure.

Here's what it looks like. This is right in line what everything that Lacey has been talking about. But he, go you deep nerd dive. Looks like it. Looks like it.

Into the depths. He's an amazing, amazing, doctor. So I just wanted to mention that to you if you are interested. Just a little little extra, nudge. Okay.

How does the thyroid impact motility and peristalsis? Oh, yeah. There's a huge there is a gut thyroid, connection, and we do have some education around that. Well, of course, hypothyroid issues are linked directly to motility issues and constipation for women, so it's it's it's quite unfortunate. With with our program program and protocols, we do see changes in some of the the gut hormone links.

We don't have any direct evidence linking it to the thyroid, but in theory, it would, support thyroid health as well, especially because it's supporting liver health. And a lot of liver or thyroid hormone production starts in the liver too. So we see this this entire, you know, network of connection between our bodily organ systems, and when they're all working optimally, you'll have better results. Now, if you have motility issues, while also having a thyroid condition, reconditioning the microbiome can help. You You may need a little extra help.

We could do utilize things like, potent gingerwalls that help with motility. Even a little bit of magnesium citrate can help give you relief. We don't wanna rely on them, but we also don't want you to feel uncomfortable on a day to day basis. I've taken literally dozens of courses of antibiotics. How do I rehabilitate my gut that I basically napalmed most decades of my life to a meaningful degree?

Well, you're not alone. I think that so many people have been exposed to antibiotics throughout the course of their life. And in fact, I have Sarna throat right now for the first time in my Oh my gosh. Life. I know.

I know. So, you know, none of us are completely immune. I neglected it for a week and a half, but now I have to be on penicillin. So, you know, I can't we can't control everything. Right.

But you can do the best you can, so, you know, give your body the tools it needs. Does that mean you're gonna regrow everything from what you were born with? Probably not. We we can't even answer that question, but we do think that the microbiome has a memory and it knows what it wants. Now, we can't tell you exactly what needs to go in your gut, but we can give it the right tools that it needs to rebuild.

So I would start with especially megaspore, megapre, give it tons of megapre and see what types of organisms can regrow. In the future, hopefully 10, 15 years down the line, we'll have more information on fecal microbiome transplants, and that may be a solution. Today, we don't have that information readily available. And thanks for doing this with strep throat. I'm so sorry.

Oh, it's okay. I feel way better. Don't worry. Okay. I'm vegan, so I cannot take megamucosa.

What can I do or take to serve a similar function? Hypotunia. Yeah. The the citrus polyphenols are something that you can do and then of course the amino acids as well. But we do get that question sometimes.

There's nothing from the binding capacity that I'm thinking about because that is that's the cow source, so that's the only animal protein source, but if you find a citrus polyphenol blend, that can help. But then trying to think other things within the vegan diet that can play a role in supporting your costal barrier health. I would cause barrier health. I would also just Google, like, vegan IgG. There's some from no.

That won't work. I know. Something about collagen and bone broth, and I'm like, nope. Nope. Nope.

And Yep. We have to keep going. I don't know why. Yep. We don't have a we don't have an easy answer on that one.

Potent gingerwalls, is that what you said? Yes. I can help with motility. Mhmm. Okay.

So that's so ginger. Yeah. I don't I don't like to use, like, cape aloe too often. It's it's very triggering and and, aggravating to the the gut. Jean, she's asking, could you go over again how much to take for the total of the 12 weeks?

I wanna show you something because we have so many questions. We're not gonna be able to do that right this second. But when you go to the link, that I posted, you can actually, hang on. You can where did it go? You literally go Total Gut Restore.

It takes you to microbiome labs and it has the instructions on the website. So I wanted to to mention that. And when you when you pick it up at Microbiome Labs, it is here it is. I got it. Okay.

It it it so here's what it looks like. It comes in a box. Recondition, reinforce, rebuild. You have another little presentation there. It talks about each product, and then it teaches you how to use it.

Yeah. So it comes in a kit. Yep. Oh, there you go. Alright.

And we did that. Okay. I'm having a colonoscopy soon. What sorts of things should I have them look for, get pictures of, would help me to know if these products you're showing us would direct help for me? Well, there's no Or no.

Yeah. Yeah. It's a bummer because we think they're gonna have a tube down there. They're gonna be able to see if I have leaky gut, and they can't. That isn't what there's really very few tests for leaky gut.

What is your favorite test for leaky gut? For leaky gut, so the I would say there's there's 2 different tests. You can do a leaky gut test through, Vibrant Clinical Laboratory. They have an excellent leaky gut test through the gut zoomer test. I'm Sarna.

Wheat zoomer wheat zoomer test. Mhmm. But then if you wanna look at the microbiome, we offer a microbiome testing, solution called BiomeFX, and this allows us to look at the types of bacteria, keystone species, how many bacteria contain lipopolysaccharide, and then the functions of your gut. So we can tell you if you're creating methane, hydrogen sulfide, different inflammatory compounds, and it gives us a a snapshot of what's going on in there. Hey.

A colonoscopy is one of the most effective medical, procedures that you can have done to protect yourself. So it's a really good procedure. But if you wanna look at your microbiome, not gonna be the right test for that. So And if you did wanna get a microbiome test at, Microbiome Labs, which has the very advanced technology and testing, that's also 15% off too. Okay.

In what ways exactly does travel pose a dysbiosis risk? At what point of travel, where and how? Yeah. It's just the the the stress, the changes in in, your sleep patterns, the foods that you're eating, the antibiotics that you're exposed to on the United Airlines flight where it's sprayed down with an antimicrobial substance. Sarna it's all these little things that just play a role in it, including even radiation in the sky when we're traveling.

Yeah. So there's a lot of And potential food poisoning Yeah. As well as I just had it. Oh, have you ever heard of, vacation constipation? Oh, yeah.

Every woman on the planet has heard of that one. So definitely Sarna avoid food food food poisoning. And I know no one needs to live in a bubble. It's just can arm ourselves with these tools to prevent it from getting worse and to get ourselves back on track. Okay, guys.

If you go to the microbiome website, microbiome labs website, and you look for the stool test, you'll find it there. It's called BiomeFX. Is that right? Mhmm. Yep.

BiomeFX as in f as in Frank, x as in xylophone. Mhmm. Okay. What do you or the literature consider too heavy exercises and too much exercise that results in gut leakiness? Where is this line with types, duration, frequency of exercise?

Also would appreciate knowing the same with the numbers of hours of sleep. Sleep is a little bit easier for me to, talk about. It's it's consistently less than 6 hours of sleep per night. Mhmm. Yeah.

That's a hard one for me too. I have a 1 year old and a busy life, and my aura ring yells at me every single morning. But, exercise is a little Mark difficult for me to quantify. Now if you're an avid marathon runner and you're doing marathon after marathon marathon, that's probably a a lot of training. It does put a lot of stress on our body.

You know, health is like a a bell curve. So we have, you know, a really good amount of exercise is probably around 60 minutes a day. When we start doing these extremes a couple of hours a day, it it leads to it being too much. It's a lot on our joints. It's a lot on our body.

It's a lot on our gut. So that extra exercise, those long runs that marathon runners will do, I've run many marathons, it does lead to a more permeability in the gut, as a way to facilitate more nutrient intake, water flow, and absorption. It's your body's way of trying to compensate for such a stressful state. I would say that IgG mixed into a water bottle, I didn't know about this, but I used to do marathons, can be really beneficial if you mix that powder into a a a performance drink. Okay.

We have 12 questions and we have 8 minutes. Let's see what we can do. Are there certain things we can take that effectively bind the lipopolysaccharides and get them out of the body that way? IgG. IgG, number 1.

Colostrum cannot compete at all. Colostrum has, like, 8%. And what can a person take that has been shown to bind the endotoxins other than the LPS, other than dietary fiber? Again, the IgG, the immunoglobulins coming from, calcium. I know that sounds gross.

It's extremely clinically effective Siebecker by, like, 60 trials and highly regulated. It's amazing. Is LPS related to beta glucuronidase activity being off as well? How do these affect the menstrual cycle specifically? Yes and no.

So beta glucuronidase is a way of, showing us if you're recycling too much estrogen, so for those in the audience. So it's gonna let us know if there's a a playing role in estrogen dominance, things like PCOS. Now the same bacteria that contain LPS are the same bacteria that secrete beta glucuronidase. So there is a link there. It's not the same.

But if you lower 1, you're probably lowering the other. Absolutely. Any research done on LPS levels changing with semaglutide and other injections? No. That would be too new, but that's an interesting idea.

And I would guess that if you have high LPS, you're not gonna get the effect that you want from the semaglutides or GLP one agonists. And we hear that a lot of people aren't, risk, like, getting the results that they want, and that can be from a million reasons, but yeah. Mhmm. How does a traumatic brain injury affect the gut Then how to restore the motility of the gut after it suffers greatly from a tibia or other? I'm so sorry.

Yeah. And that and that's very difficult. You'll probably have a leakiness and a permeability, Mark permeable, blood brain barrier following a traumatic brain injury. I would support the gut with just a total gut restoration protocol. Watch your diet, eat organic, and and let your body heal itself.

I mean, there's there's some Take it easy. Look in look into melatonin. Also, there's some good advice. I'm still thinking about the coconut oil LPS connection. Who should consider consuming coconut and other oils, and who should absolutely avoid taking it?

Yeah. It's really looking at the overall composition of your diet. I'm not somebody that says, no. You can't do that. But, when we look at the LPS spike, we see a higher spike with coconut oil than we do with something like olive oil.

So just using the right oils for the right things that we're cooking with. I love steamed caras with coconut oil. You can't tell me not to do that. I think it tastes so good together. But if you're eating a very meat and fat heavy diet, then you're gonna have to watch your composition, compared to somebody that has more of a foundation in vegetables, plants, and fruits.

My alternative practitioner this is a good one. My alternative practitioner told me that spore probiotics are basically a bottle of micro dirt that has any excrement from animals and other things filtered out. And that can we can get the same thing from eating vegetables from the garden with dirt on it. Well, don't eat don't eat dirt because you can get other things in like, you can get botulism Earth sites. Parasites.

Just the first one, I can't do that. That's a very limited ignorant view. I'm sorry. It is. It is.

That's a very strong statement for me to say. I wouldn't say things like that. Yeah. The how were spore biotics discoverable? They were discovered when, people were actually eating camel dung during World War 2, when they had GI issues in the desert, and they later on figured out what was helping their GI distress, they are found in the nature around us, which is good.

We want to utilize bacteria that can survive in the world around us. So our ancestors consumed them, we consumed them. But we've combined the combination of nature and science. We've isolated out the spores and the strains that have the biggest benefit to you. And then they studied it like crazy.

We're moving forward. Crazy. Like, we just watched a half an hour presentation, not over 40 minutes on it. Don't need dirt. Yeah.

Yeah. Okay. Okay. I'm assuming if one discontinues your product, the results come to an end until the daily dose continues. Could be wrong.

Well, I mean, your opinion. How long do the effects of your products last from start to finish? That depends on your microbiome, of course. And are there any cumulative results that last for months or years after one chooses and discontinues the products? We haven't done a longitudinal study that would go on for years years.

But what I could say is it just depends on your overall diet and lifestyle. If you're someone like, hey, I wanna party every weekend, and I wanna eat my fast food. I'm just gonna be on these products for the rest of my life. Fine. So be it.

But if you wanna try to eat an organic diet, reduce your toxic burden in the household, then I think you're somebody that can move on to a maintenance program. And, if you're working it depends on your symptoms too. If it's got brain, work on meditation. If it's do you see what I mean? Yes.

Of course. Contextual. Yes. For people taking h two blockers, how does this impact the acidic environment promoted by mega prebiotic hygiene? They won't play a role at all.

So if you're taking any sort of antacids, you're talking about the stomach, which is a good 7, 8, 10 feet away from the colon. It's a totally different world. When you leave the small intestine and go into the colon, you go through a Allison it's a different environment. So it's it's like outer space. That's how I think of it.

So totally different, won't impact it. Okay. If you're on an h two blocker though, you do wanna recondition your gut because oftentimes we see more pathogens get through if your stomach acid is low. How much can we increase the mucosal layer with pre or probiotics relative to products like marshmallow leaf, or root? I don't know enough of the research if there's been enough research done on the actual mucus membrane.

Of course, those are really gentle and soothing herbs that can play a role in overall digestive health. We're very research Siebecker, so that's just where my education lies there. But doesn't one need adequate HCL to break down the capsules? I mean, you can argue that, but you can just take it out of the capsule. Yeah.

You don't need it in the capsule. There's no function. Some people use the the the delayed release. We don't need that. Right.

Sorry to say this. I have a lot of dampness, snottyness, etcetera, that I deal with every day. Is that a sign of thick mucosal barrier, perhaps too thick with too much mucus? Not necessarily. If you have a lot of dampness and sinus, I'd be worried about your just upper respiratory health, and you might wanna look into something like nasal lavages and stuff like that.

But, no, it shouldn't have any impact over your overall gut health. The the the same cells, but different parts of your body. Mhmm. Over what period of time does endotoxemia develop? Typically days, weeks, months, or years.

And how long does it take to reverse the endotoxemic state as soon as how long? And Pam, go to back to the link. You don't have the whole link. That's why your link isn't working. Go back to the chat and you will find it there.

I literally am staring at the full link on there. Okay. So what period of time does it take to develop endotoxemia? Well, I would argue that it can happen kinda quickly, not to be scary, but, you know, over the course of, let's say, a month or so. If you've remember that movie, Super Size Me, where you ate McDonald's every single day for 30 days, you know, I would argue that at the end of that 30 days, he was probably in a very metabolic endotoxemic state with high LPS because you're just going through such an extreme.

But the beauty is the result You got rest of soul by the way. I know. As I was saying that, I I remembered that. But your your microbiome is flexible. Look at our WikiCAD study.

Call students re eating pizza. After 30 days, we reduce metabolic endotoxemia by 42%. So it's not about living all this restriction and fear. It's about healing. We can we can heal.

Yes. We can heal. Jean, are the products or kits compatible with SIBO protocols? No. No.

They're not. Okay. Thank you. Sarna, what? You wanna get that SIBO reduced?

You wanna get that overgrowth reduced? Get yourself that microbiome balanced out in terms of prebiotics. You don't wanna do that until that SIBO is resolved. A lot of people do beautifully with MegaSpore. The other parts of the kit, you know, start slow and build up.

But for the prebiotic, you wanna wait until your SIBO is cleared. Agreed. Yes. Make sure that's totally clear. Make sure you're out of the window for a relapse before you weave in the prebiotic.

Now I think it's essential though once you're in the clear and everything is functioning properly because you probably need some rebuilding further down. Thank you, Lacey. Thank you, everyone. Thank you so much for being here. Thanks for joining me late in the day for our usual, 7:15:45 minutes from now.

If you do send us your proof of purchase for anything at Microbiome Labs, that you've bought today with our link, Someone sent me something from, like, a week ago from someone else's account. I was like, what? Yeah. Anyway, so send that to us at info@sbosos.com, and we will send you that phenomenal master class. Info at Cbosos, if if you can pull that off.

If not, you're still getting the 15% off the entire microbiome labs cart, including the vaginal microbiome kit and the stool microbiome kit, tests, I should say. They're really phenomenal. Lacey, I'm gonna send you a different link so we can just do a recap, and, thanks everybody. Alright. Have a great well, not everything.

Alright. We'll send you a replay as well. Thank you so much. Alright. Great job.

Great job, everybody. Thanks. Bye.

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